Throneberry bimolecular: concealment return

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– A high unspecific binding response concealed the specific binding response when we used HBST as running buffer (a commonly used buffer in Biacore assays) in which we had studied DKK1 and DKK4. To minimize unspecific binding of our His-tagged DKK2, which had a theoretical net charge of +23 at pH 7.0, we chose a kosmotropic buffer, as used by.

Throneberry bimolecular: concealment return Upon return to 37 C, lysozyme assumes its native fold. 28 The ability of oligomers and protofibrils to seed fresh growth at 37 C, combined with the inability of filaments to do so, implies the absence of even small percentages of partially unfolded solution states which would have supported continued.

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